The approximate molecular weights of polysaccharides are measured using size exclusion chromatography (SEC). The molecular weights of oligosaccharides are measured by MALDI-MS, either non-derivatized or after derivatization.
Molecular Weight Determination and Purification of Polysaccharide by HPLC
Molecular weight determination of polysaccharide by size exclusion chromatography; purification of the polysaccharide according to its molecular weight for further structure analysis (glycosyl composition, glycosyl linkage or NMR, et al)
High performance liquid chromatography (HPLC) is used for size exclusion chromatography (SEC) using Refractive index (RI), U.V or ELSD or fluorescent detectors.
Amount of Sample Required for Analysis
If you have no knowledge of the nature of the sugar constituents of your sample and you desire an ideal analysis, a minimum of 500 micrograms of carbohydrate is required. If you have considerably smaller amounts of carbohydrate sample, we can make special arrangements to determine the composition of your sample. Please contact us to discuss your specific requirements, and do not let limited sample amount deter you from requesting our services.