Full characterization of a target protein’s glycosylation includes N– and O-glycoform structures, mapping of glycosylation sites and extent of glycosylation at each site.
Analysis of peptides and glycopeptides produced following protease digestion is readily achieved by LC-ESI-MS/MS or nano-LC-NSI-MS/MS with the CCRC Analytical Service’s Thermo LTQ- Fusion Tribid and LTQ-Velos instrumentation.
Electron transfer dissociation (ETD) and high collision dissociation (HCD) fragmentation capabilities enhance glycopeptide analysis and site of glycosylation studies.
While most glycopeptide analyses are completed by manual data interpretation, numerous software such as Thermo Proteome Discoverer™ and Protein Metric’s Byonic ™ are used supplementary for our data analysis.
