Instrumentation
The Analytical Service and Training Laboratory is a unique facility with state-of-the-art instrumentation designed for detailed structural elucidation of complex carbohydrates.
We have MALDI-TOF and MALDI-TOF/TOF and electrospray and nanospray ionization techniques coupled with ion trap and Orbitrap mass analyzers including a thermo fusion tribrid mass spectrometry instrument. We perform tandem mass spectrometry using CID, HCD and ETD fragmentation and ion activation mechanisms for compositional and sequence analysis of N and O linked glycans and glycopeptides.
One dimensional and two dimensional Nuclear Magnetic Resonance spectroscopy is used for the comprehensive structural analysis and repeating unit sequencing of polysaccharides and the full structural characterization of polysaccharides and glycans. Non carbohydrate substituents and molecular carbohydrate protein interactions can also be determined.
Mass Spectrometry
| Instrumentation | Number |
|---|---|
| Thermo Orbitrap Ascend Tribid with Nano HPLC and UVPD | 1 |
| Waters Synapt XS HDMS with UPLC-FLD and Ion Mobility | 1 |
| Thermo Orbitrap Eclipse Tribrid with Nano-HPLC and FAIMS | 1 |
| Thermo Orbitrap Fusion Tribrid with Nano-HPLC | 1 |
| Thermo Velos-Orbitrap-Elite with UHPLC | 1 |
| Thermo LTQ-Orbitrap Discovery with Nano-UHPLC | 1 |
| Shimadzu IT-TOF | 1 |
| Agilent GC-MS with EI Source | 7 |
| Agilent GC-MS with Pyrolysis Unit | 1 |
| Shimadzu Ultra GC-MS with CI Source | 1 |
| Extrel Pyrolysis Molecular Beam MS | 1 |
| ABI Sciex 5800 MALDI-TOF-TOF | 1 |
| Bruker rapiflex Tissuetyper MALDI-TOF | 1 |
| HTX M3+ MALDI Sprayer | 1 |
| Bruker Microflex LRF MALDI-TOF | 2 |
Chromatography
| Instrumentation | Quantity |
|---|---|
| ӒKTA pure 25 L1 Protein Purification System | 1 |
| Agilent HPLC FL detector | 1 |
| Waters Acquity UPLC-FLD | 1 |
| Agilent HPLC MALS detector | 1 |
| Agilent HPLC UV detector | 3 |
| Dionex HPAEC with PAD Detection | 2 |
| Dionex ICS-6000 | 1 |
| Sciex Capillary Electrophoresis system | 2 |
NMR Spectrometers
| Instrumentation | Number |
|---|---|
| Bruker Avance Neo 900 MHz | 1 |
| Bruker Avance Neo 800 MHz | 1 |
| Bruker Avance Neo 600 MHz | 1 |
| Bruker Avance III 600 MHz | 3 |
Others
| Instrumentation | Quantity |
|---|---|
| Thermo Scientific Orbitor RS with Biotek Multiflo, EL406, EON and Precision XS | 1 |
