Instrumentation
The Analytical Service and Training Laboratory is a unique facility with state-of-the-art instrumentation designed for detailed structural elucidation of complex carbohydrates.
We have MALDI-TOF and MALDI-TOF/TOF and electrospray and nanospray ionization techniques coupled with ion trap and Orbitrap mass analyzers including a thermo fusion tribrid mass spectrometry instrument. We perform tandem mass spectrometry using CID, HCD and ETD fragmentation and ion activation mechanisms for compositional and sequence analysis of N and O linked glycans and glycopeptides.
One dimensional and two dimensional Nuclear Magnetic Resonance spectroscopy is used for the comprehensive structural analysis and repeating unit sequencing of polysaccharides and the full structural characterization of polysaccharides and glycans. Non carbohydrate substituents and molecular carbohydrate protein interactions can also be determined.
Mass Spectrometry
| Instrumentation | Number |
|---|---|
| Thermo Orbitrap Fusion Tribrid with Nano-HPLC | 1 |
| Thermo Velos-Orbitrap-Elite with UHPLC | 1 |
| Thermo Orbitrap Eclipse Tribrid with FAIMS and Nano-HPLC | 1 |
| Thermo Q Exactive HF Hybrid Orbitrap with UHPLC | 1 |
| ABI Sciex 5800 MALDI-TOF-TOF | 1 |
| Bruker Microflex LRF MALDI-TOF | 1 |
| Shimadzu Ultra GC-MS with CI Source | 1 |
| Agilent GC-MS with EI Source | 5 |
| Quantum Pyrolysis, Extrel Molecular-Beam-MS | 1 |
| Quantum Pyrolysis, Agilent GC-MS | 1 |
| Beckman Coulter CE 8000SI Interfaced to Thermo LTQ-MS | 1 |
Chromatography
| Instrumentation | Quantity |
|---|---|
| Shimadzu Ultra GC-FID | 1 |
| Dionex HPAEC with PAD Detector | 2 |
| Agilent HPLC with UV, Fluorescent, RI, ELSD and CAD Detectors | 5 |
| Beckman Coulter CE PA800 with UV, PDA LIF (488 em, 570 ex) | 1 |
NMR Spectrometers
| Instrumentation |
|---|
| Bruker NEO 900 MHz-NMR |
| Bruker NEO 800 MHz-NMR |
| Bruker NEO 600 MHz-NMR |
| Bruker Avance III 600 MHz-NMR |
| Varian DD 600 MHz-NMR |
| Varian/Agilent DD 600 MHz-NMR |
| Varian Inova wide-bore 500 MHz-NMR |
| Varian Mercury 300 MHz-NMR |
Others
| Instrumentation | Quantity |
|---|---|
| Thermo Scientific Orbitor RS with Biotek Multiflo, EL406, EON and Precision XS | 1 |
