Course 3
Analytical Techniques for Structural Analysis of Glycosaminoglycans (GAGs) (August 17-19, 2025)
Monday, August 17, 2026
9:00 a.m. – 9:15 a.m.
Introduction and Welcome
Dr. Parastoo Azadi
9:15 a.m. – 10:30 a.m.
Lecture – “GAG Analysis Methods, Part 1“
Dr. Christian Heiss
10:30 a.m. -10:45 a.m. – Break
10:45 a.m. – 11:15 a.m.
Lecture – “GAG Analysis Methods” (continued)
Dr. Christian Heiss
11:15 a.m. – 12:30 p.m.
Laboratory
Introduction to Laboratory experiments
Experiment 4 – Nitrous Acid Preparation and Digestion. Page 9.
Prepare samples for analysis by HPLC.
Dr. Stephanie Archer-Hartmann
Laboratory
Introduction to Chromatographic Methods – SAX-HPLC introduction. Page 9.
Dr. Stephanie Archer-Hartmann
12:30 p.m. – 1:30 p.m. – Lunch
1:30 p.m. – 2:30 p.m.
Laboratory
Experiment 4 – Discussion of Experiment 4 Results. Discussion of alternative methods
Dr. Stephanie Archer-Hartmann
2:30-3:00 p.m. –
Lecture “Considerations for GAG Isolation from Cells and Tissue”
Dr. Stephanie Archer-Hartmann
3:00 p.m. – 5:00 p.m.
Laboratory
(Pre-Experiments 1-3) – Introduction of Analysis by Enzymatic Methodologies.
Start Enzyme Digestions. Page 3
Dr. Stephanie Archer-Hartmann
Experiment 5 – Sulfate Analysis – Introduction
Hydrolysis. Page 13.
Dr. Stephanie Archer-Hartmann
Tuesday, August 18, 2026
8:45 a.m. – 9:00 a.m.
Questions and Discussion
9:00 a.m. – 10:00 a.m.
Laboratory
Experiment 2 – Stop Enzyme Digestions
Prepare Sample and Inject on SAX-HPLC. Page 6.
Experiment 3- Prepare centrifuge tube to dry down. Page 8.
Dr. Stephanie Archer-Hartmann
10:00 a.m. – 10:30 a.m. – Break
10:30 a.m. – 12:00 p.m.
Experiment 5 – Sulfate Analysis – Plate Assay. Page 13.
Dr. Stephanie Archer-Hartmann
12:00 p.m. – 1:00 p.m. – Lunch
1:00 p.m. – 2:00 p.m.
Demonstration – “Optical Analytical Techniques: Surface Plasmon Resonance (SBR) and Biolayer-Interferometry (BLI).”
Dr. Varughese Mulamoottil
2:00 p.m. – 2:15 p.m. – Break
2:15 p.m. – 3:45 p.m.
Lecture – “Glycosaminoglycans in Biomedicine”
Dr. Ryan Weiss
3:45 p.m. – 5:00 p.m.
Laboratory
Experiment 3 – Introduction to GAG Disaccharide Labels
Label with AMAC (Demonstration). Page 8.
Dr. Stephanie Archer-Hartmann
Experiment 7 – Introduction to SEC: MW Determination of whole GAGs and GAG products by SEC-HPLC. Page 15.
Dr. Stephanie Archer-Hartmann
Wednesday, August 19, 2026
8:45 a.m. – 9:00 a.m.
Questions and Discussion
9:00 a.m. – 10:30 a.m.
Lecture – “Intro to NMR for Glycosaminoglycans”
Dr. Christian Heiss
10:30 a.m. – 10:45 a.m. – Break
10:45 a.m. – 12:30 p.m.
Laboratory
Experiment 1 – Data Analysis and Interpretation
Dr. Stephanie Archer-Hartmann
Experiment 6 – Data Analysis and Interpretation
Dr. Stephanie Archer-Hartmann
12:30 p.m. – 1:30 p.m. – Lunch
1:30 p.m. – 2:15 p.m.
Laboratory
Experiment 2 – Data Analysis and Interpretation
Dr. Stephanie Archer-Hartmann
2:15 p.m. – 2:30 p.m. – Break
2:30 pm – 3:30 p.m. –
Lecture: “Carbohydrates Drug Products and their Structures”
Dr. Parastoo Azadi
3:30 p.m. 4:15 p.m.
Laboratory
Experiment 3 (DEMO) – Separation of AMAC Labeled GAGs by Capillary Electrophoresis and UHPLC-HPLC. Page 8.
Dr. Stephanie Archer-Hartmann
4:15 p.m. – 5:00 p.m.
Lecture:– “Monosaccharide Composition and Linkage by GC-MS”