Mass Spectrometry Analysis Using Electrospray and Nanospray Ionization
We provide high resolution high sensitivity mass spectrometry analysis using electrospray and nanospray ionization, Matrix Assisted Laser Desorption Ionization (MALDI) with time of flight (TOF) as well as iontrap and orbitrap mass analyzers.
MALDI allows the determination of the molecular weight of oligosaccharides, glycopeptides, glycoproteins, and other glycoconjugates.
Amount of sample required for analysis: If the sample is a singly purified glycoprotein, we need a minimum of 500 pmol of material to produce a clear result, more if the sample is a crude protein.
General criteria for sample preparation: Your sample should be pure, soluble in water or volatile organics, and free of non-volatile salts, buffers, and detergents. MALDI-MS can be performed on ABI 5800 or Bruker Microflex on sample up to about 150kDa.