Routine Services

Mass Spectrometry Analysis Using Electrospray and Nanospray Ionization

We provide high resolution high sensitivity mass spectrometry analysis using electrospray and nanospray ionization, Matrix Assisted Laser Desorption Ionization (MALDI) with time of flight (TOF) as well as iontrap and orbitrap mass analyzers.

MALDI-TOF-MS

MALDI allows the determination of the molecular weight of oligosaccharides, glycopeptides, glycoproteins, and other glycoconjugates.

Amount of sample required for analysis: If the sample is a singly purified glycoprotein, we need a minimum of 500 pmol of material to produce a clear result, more if the sample is a crude protein.

General criteria for sample preparation: Your sample should be pure, soluble in water or volatile organics, and free of non-volatile salts, buffers, and detergents. MALDI-MS can be performed on ABI 5800 or Bruker Microflex on sample up to about 150kDa.

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) graph

Electrospray/Nanospray Ionization Ion-trap Mass Spectrometry (ESI/NSI-MS and MS/MS )

Electrospray and nanospray Ionization is combined with ion trap, Orbitrap or TOF analyzers and is used for structure elucidation of oligosaccharides, glycopeptides, glycoproteins, and proteins.

Amount of sample required for analysis: If the sample is a singly purified glycoprotein, we need a minimum of 500 pmol of material to produce a clear result, more if the sample is a crude protein.

General criteria for sample preparation: Your sample should be pure, soluble in water or 50% ethanol, and free of non-volatile salts, buffers, and detergents.

On-Line Liquid Chromatography-Electrospray Ionization-Mass Spectrometry (LC-ESI-MS)

Sites of N- and O-linked glycosylation, glycopeptide analysis and peptide mapping are performed by LC-ESI-MS-MS.

Amount of sample required for analysis: A minimum of 100 picomoles of peptides or glycopeptides is required.

Electrospray/Nanospray Ionization Ion-trap Mass Spectrometry (ESI/NSI-MS and MS/MS ) graph