Techniques

High Performance Liquid Chromatography

High Performance Liquid Chromatography (HPLC) is a fast, robust analytical technique with a wide array of application in the laboratory.

• HPLC is frequently used for size exclusion chromatography (SEC) of polysaccharides and glycosaminoglycans (GAGs) to determine molecular weight, separation and isolation of carbohydrates by size and detected by RI and/or ELSD.
• With CTA-SAX column, HPLC is used to determine the distribution of low molecular weight heparin (LMWH) as an indicator of extent and pattern of depolymerization of heparin.
• HPLC is also used for analysis of disaccharides from GAGs with a UV detector followed in sequence by post column derivatization with a fluorescent detector.
• With appropriate column, we use HPLC to analyze, separate, isolate and/or purify peptides and glycopeptides including preparation for downstream analysis like mass spectrometry.