The absolute configurations of monosaccharides are determined by GC-MS analysis. Identification of D and L forms is made by comparison of retention time to standard D and L monosaccharides.
Absolute configurations (D or L) of Monosaccharides
Purpose: Determination of the absolute configurations (D or L) of monosaccharides
Amount of sample required for analysis: A minimum of 200-500 micrograms of carbohydrate is required, more if available.
Methods used: The absolute configuration of monosaccharides can be determined by acid-catalyzed derivatization with optically pure 2-butanol, followed by per-O-trimethylsilylation and GC-MS analysis. Identification of the D and L forms is made by comparison of retention times the standard D or L of monosaccharides.