We offer analyses for quantification of cellulose and hemicellulose in lignocellulosic biomass providing expertise in:
- Fractional Extraction of Hemicelluloses – Cellulose and some associated polymers will remain insoluble after extraction of hemicelluloses. Isolated hemicellulose fractions are used for glycosyl composition, glycosyl linkage (PMAA), MW distribution (SEC), and structure elucidation
- Phenol-Sulfuric Colorimetric Assay – For cellulose residue after removal of hemicelluloses. Phenol-Sulfuric assay is a basic colorimetric assay to quantify neutral sugars in polysaccharides or mixtures. Sample amount required: at least 100mg of the complex mixture.
- Glycosyl Composition and Linkage – We can provide qualitative and quantitative monosaccharide composition analysis (At least 100-500 micrograms of sample is used for this analysis). In addition permethylation analysis resolves glycosidic linkages between sugar residues and is very useful tool in characterization of complex plant glycans 200mg up to 1mg or higher amounts of sample is required for linkage analysis.
- 1D- and 2D-NMR spectroscopy – 1D and standard 2D homo- and heteronuclear nuclear magnetic resonance spectroscopy (NMR) is an extremely powerful technique for determination of carbohydrate sequence and structure of plant poly- and oligosaccharides. It also helps to evaluate sample quality, – α/β – anomeric configuration of glycosyl residues, and quick assessment of the nature of purified polysaccharide. Information on sample requirements can be found at: NMR Facilities
- Glycosyl Composition – We can provide qualitative and quantitative monosaccharide composition analysis of the biomass (At least 100-500 micrograms of sample is used for this analysis).