Glycomics/Glycoproteomics

Milk Oligosaccharides

We can isolate and analyze milk oligosaccharides (MOs) from different sources of raw milk (human, primate, bovine, etc).  Purified MOs are derivatized in preparation for MS analysis:

Milk Oligosaccharide Profiling by MALDI-TOF-MS

Permethylated samples can be analyzed by MALDI-TOF-MS to obtain oligosaccharide profiles as well as some structural information on low molecular weight MOs by MS/MS fragmentation.

MALDI-TOF-MS

Structural Characterization by LC-NSI-MS/MS

Permethylated MOs can be separated and analyzed in higher detail by means of LC-NSI-MS/MS. This analysis can provide sequencing information, structural elucidation of MOs, relative quantitation of glycoforms and milk group identification (secretor status / Lewis epitope) by means of MS/MS fragmentation. In addition, we also offer enrichment and detection of high molecular weight sialylated species.