Course 1
TECHNIQUES FOR CHARACTERIZATION OF CARBOHYDRATE STRUCTURE OF PLANT AND MICROBIAL POLYSACCHARIDES – POLYSACCHARIDE COURSE (August 3-7, 2026)
Monday, August 3, 2026
9:00 a.m. – 9:15 a.m.
Welcome and Introduction
Dr. Parastoo Azadi
9:15 a.m. – 9:30 a.m.
Hydrolysis of AA samples
9:30 a.m. – 10:15 a.m.
Lecture – “Structural Characterization of Carbohydrates” (Part 1)
Dr. Parastoo Azadi
10:15 a.m. – 10:30 a.m. – Break
10:30 a.m. – 11:00 a.m.
Lecture – “Structural Characterization of Carbohydrates” (Part 2)
Dr. Parastoo Azadi
11:00 a.m. – 12:15 p.m.
Remove AA samples from heat and dry down with nitrogen
Sodium borodeuteride reduction of AA samples
Preparation of Size Exclusion Samples
12:15 p.m. – 1:15 p.m. – Lunch
1:15 p.m. – 2:30 p.m.
Neutralization and drying of AA samples
Acetylation of AA samples
2:30 p.m. – 3:45 p.m.
Lecture – “Chemical Properties of Mono and Oligosaccharides”
Dr. Geert-Jan Boons
3:45 p.m. – 4:00 p.m. – Break
4:00 p.m. – 5:00 p.m.
Drying, extraction, and injection of AA samples
Overnight methanolysis of acetylated and native methyl glycosides (TMS) samples
Tuesday, August 4, 2026
9:00 a.m. – 9:30 a.m.
Dry down methyl glycosides (TMS) samples
Prepare NaOH base for permethylation (Linkage)
9:30 a.m. – 10:25 a.m.
Permethylation of the linkage samples
N-acetylation of methyl glycosides samples (TMS)
10:25 a.m. – 10:40 a.m. – Break
10:40 a.m. – 11:15 a.m.
Remove the CH3I, DCM Extraction and drying (Linkage)
Drying of the N-acetylated methyl glycosides (TMS) samples after 30 min reaction
11:30 a.m. – 12:30 p.m.
Lecture – “Bacterial Capsular Polysaccharides: Structure, Immune Roles and Analytical Techniques in Vaccine Development”
Aidan Keelaghan
12:30 p.m. – 1:45 p.m. – Lunch
1:45 p.m. – 3:15 p.m.
Trimethylsilylation of the methyl glycosides- (TMS) samples
Drying, centrifugation, and injection of the methyl glycosides- (TMS) samples
Demonstration– Preparation of (dimsyl) potassium dimethylsuphonyl anion
3:15 p.m. – 4:30 p.m.
Lecture – HPAEC
Dr. Parastoo Azadi
4:30 p.m. – 4:35 p.m. – Break
4:35 p.m. – 4:45 p.m.
Overview of GCMS instrument
Dr. Ian Black
4:45 p.m. – 5:00 p.m.
Lecture – “FACES Scheduling”
Saeid Roushanzamir / Brian Perkins
Wednesday, August 5, 2026
9:00 a.m. – 9:15 a.m.
Hydrolysis of PMAA samples
9:15 a.m. – 10:00 a.m.
Demonstration – Lignin Analysis by Pyrolysis -Molecular Beam -MS
Dr. Artur Muszyński
10:00 a.m. – 10:15 a.m. – Break
10:15 a.m. – 11:30 a.m.
Lecture – “Isolation and Characterization of Bacterial Surface Polysaccharides”
Dr. Artur Muszyński
11:30 a.m. – 12:15 p.m.
Remove linkage samples from heat and dry down with nitrogen
Sodium borodeuteride reduction of linkage samples
12:15 p.m. – 1:15 p.m. – Lunch
1:15 p.m. – 2:30 p.m.
Neutralization and drying of PMAA samples
Acetylation of PMAA samples
2:30 p.m. – 3:15 p.m.
Data analysis of AA
Drs. Muszyński and Black
3:15 p.m. – 3:30 p.m. – Break
3:30 p.m. – 4:15 p.m.
Lecture – “Labeling, Preparative and Analytical Size Exclusion Chromatography of Polysaccharides- a practical approach for isolation and purification of polysaccharides”
Dr. Stephanie Archer- Hartmann
4:15 p.m. – 5:00 p.m.
Drying, extraction, and injection of PMAA samples
Thursday, August 6, 2026
9:00 a.m. – 10:00 a.m.
Data analysis of TMS
Drs. Muszyński and Black
10:00 a.m. – 10:15 a.m. – Break
10:15 a.m. – 12:15 p.m.
Data analysis for linkage
Drs. Muszyński and Black
12:15 p.m. – 1:15 p.m. – Lunch
1:15 p.m. – 2:30 p.m.
Lecture – “Cellulose: Structure, Analysis, and Application”
Dr. Ian Wallace
2:30 p.m. – 2:45 p.m. – Break
2:45 p.m. – 3:00 p.m.
Demonstration – HPAEC -Oligosaccharide profiling analysis and monosaccharide composition analysis
Dr. Varughese Mulamoottil
3:00 p.m. – 3:30 p.m.
Data analysis for Size Exclusion Chromatography
Drs. Muszyński and Black
3:30 p.m. – 4:00 p.m.
Questions and answers for data interpretation of all the analyses
Drs. Azadi, Black, and Muszyński
4:00 p.m. – 5:00 p.m.
Lecture – “Introduction to Xyloglucan Structure and Biosynthesis”
Dr. Breeanna Urbanowicz
Friday, August 7, 2026
<Mass Spectrometry Module>
9:00 a.m. – 12:00 p.m.
Lecture – Glycomics & Glycoproteomics
Dr. Ron Orlando
10:15 a.m. – 10:30 a.m. – Break
12:00 p.m. – 1:00 p.m. – Lunch
1:00 p.m. – 3:00 p.m.:
Mass Spectrometry Facilities Tour – Mass Spec BINGO
MSMS Analysis of Glycans and Glycopeptides
Dr. Stephanie Archer-Hartmann
Or <NMR Module>
9:00 a.m. – 12:00 p.m.
Lecture – “Introduction to NMR of glycoproteins and carbohydrates”
Dr. Christian Heiss
12:00 p.m. – 1:00 p.m. – Lunch
Afternoon:
Demonstration and data interpretation
Or <Molecular Modeling Module>
9:00 a.m. – 12:00 p.m.
Lecture – “Introduction to Molecular Modeling
Dr. Lachele Foley
12:00 p.m. – 1:00 p.m. – Lunch
Afternoon:
Demonstration and data interpretation