Microbial Carbohydrates

Lipid A Structure

• Analysis of Lipid A Structure – cleavage of Lipid A Structure moiety from the O-antigen-core region of LPS by mild hydrolytic treatment followed by extensive purification, glycosyl and fatty acid composition (GC-MS); purification of lipid A via liquid-liquid extraction and HPLC; elucidation of the molecular weight and structure by high resolution MALDI-TOF MS and MS/MS, ESI-MS (MS/MS) and by 1D and 2D homo- and heteronuclear