Nuclear Magnetic Resonance (NMR)
We use both one dimensional and two dimensional nuclear magnetic resonance to determine the monosaccharide residue sequence of polysaccharides repeating units and oligosaccharides.
One-Dimensional Nuclear Magnetic Resonance
In NMR Define the types of anomeric configuration (a,b) present, and also provide information about non-carbohydrate substituents, such as acetyl or pyruvyl moieties.
This analysis also provides information about the purity of the carbohydrate. Both 13C-NMR and 1H-NMR give information regarding the number of monosaccharide residues contained in the repeating glycosyl units of a polysaccharide if it is composed of a repeating sequence. 13C-nuclear magnetic resonance can provide some information regarding glycosyl linkage and can detect the presence of unusual or rarely observed sugars.