The mass spectrometry and bioinformatics training course will focus on the analysis and data interpretation of glycoproteins by mass spectrometric techniques. MS component will include lectures on mapping the glycosylation sites in glycoproteins; determining the composition, sequencing, and branching points of N and O-linked oligosaccharides, and MS procedures used in these analyses. Demonstrations will include matrix-assisted laser desorption (MALDI), electrospray ionization (ES/MS), and tandem mass spectrometry (MS/MS). Experience with MS is beneficial but is not required.
The Bioinformatics component concentrates on the interpretation of mass spectrometric data and especially the application of bioinformatics tools for this purpose. The course is taught by Dr. Rene Ranzinger and the bioinformatics team at the CCRC with special lectures from Drs. Azadi and Archer-Hartmann. The course included lectures and hands-on exercises of:
- Introduction to different domain databases (Glycomics and GlycoProteomics) and how they can be used to supplement analytical workflows.
- Introduction in the interpretation of mass spectrometric data
- A general overview of existing mass spectrometry software tools for glycomics and glycoproteomics data
- Training in the interpretation of glycomics data using GlycoWorkbench and GRITS Toolbox
- Training in the interpretation of glycoproteomics data using Byonic™/Protein Discoverer ™
- Standards for reporting data and depositing data (e.g. MIRAGE, MIAPE)
A course manual including selected analytical techniques and references will be provided. The cost of registration (including lunch) is $500 per course for individuals from non-profit institutions and $1100 for others. The cost of travel and lodging is not included.
Course 4 Sample Schedule