To determine the positions of linkage of glycosyl residues in an oligosaccharide, polysaccharide, or glycoprotein. The linkage of the residues, including percentage of each is provided with this analysis.
Linkage analysis of neutral, uronic acids or amino-residues (methylation analysis) – The polysaccharides or oligosaccharides are derivatized prior to GC-MS analysis. This procedure uses either the Hakomori, or NaOH base, for the permethylation procedure.
Linkage analysis of Kdo– Kdo (2-keto-3-deoxy-octulosonic acid) is readily analyzed by a modified linkage procedure.
For glycosyl linkage analysis, the sample is permethylated, depolymerized, reduced, and acetylated; and the resultant partially methylated alditol acetates (PMAAs) are analyzed by gas chromatography-mass spectrometry (GC-MS).