A major goal of the Analytical Services laboratory at the Complex Carbohydrate Research Center is to stimulate collaboration with members of the broader scientific community. In addition to standard service projects, the Analytical Services laboratory also routinely works with various organizations in the form of research collaborations. This work serves to strengthen the current field of carbohydrate research, as well as to provide the feedback necessary to refine current technology and identify new needs in the field.

Collaboration projects involve our expertise in the fields of plant and microbial polysaccharides, glycomics, glycoproteomics and proteoglycans. These collaborations commonly exploit still-emerging technologies where the projects can be more open-ended and generally require a greater interaction between personnel at the CCRC and outside investigators.

Dr. Parastoo Azadi is the coordinator of Collaborative Endeavors within the CCRC Analytical Services Group. With more than 25 years of experience in the field of polysaccharide and glycoprotein structural analysis, she is well equipped to assist investigators in the development of new collaborative projects with the scientific staff at the CCRC. Initial contact may be made directly with Dr. Azadi at

Publications resulting from these projects will very likely be co-authored by all those involved in the collaboration, and any publication using data from the Resource should include an acknowledgment similar to:


Plant and Microbial Polysaccharide Research

Should any of these data be used in a publication, please include the following statement in the acknowledgment: This work was supported by the Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, U.S. Department of Energy grant (DE-SC0015662) at the Complex Carbohydrate Research Center.


Biomedical Research

The R24 grant number R24GM137782 should be acknowledged in all publications, presentations, websites, press releases, etc., generated or resulting from research or services that has been performed through the CCRC Service and training laboratory. We ask that if our scientists contribute to intellectual aspect of the project or and the manuscript preparation, they be considered as co-authors on the publication as well: The carbohydrate analysis performed at the CCRC was supported by NIH R24GM137782 grant.

Recent Publications

Barnes WJ, Koj S, Black IM, Archer-Hartmann SA, Azadi P, Urbanowicz BR, Pena MJ, O’Neill MA. (2021) Protocols for isolating and characterizing polysaccharides from plant cell walls: a case study using rhamnogalacturonan-II. Biotechnol Biofuels, 14, 142. June 22, 2021.

Shajahan A, Pepi LE, Rouhani DS, Heiss C, Azadi P. (2021) Glycosylation of SARS-CoV-2: structural and functional insights. Anal Bioanal Chem, June 22, 2021.

Supekar NT, Shajahan A, Gleinich AS, Rouhani DS, Heiss C, Chapla DG, Moremen KW, Azadi P. (2021) Variable posttranslational modifications of severe acute respiratory syndrome coronavirus 2 nucleocapsid protein, Glycobiology, May 13, 2021. cwab044,

Heiss C, Black I, Ishihara M, Tatli M, Devarenne TP, Azadi P. (2021) Structure of the polysaccharide sheath from the B race of the green microalga Botryococcus braunii. Algal Research. 55:102252. May 1, 2021.

Sun X, Wu Q, Picha DH, Ferguson MH, Ndukwe IE, Azadi P. (2021) Comparative performance of bio-based coatings formulated with cellulose, chitin, and chitosan nanomaterials suitable for fruit preservation. Carbohydrate Polymers. May 1, 2021. 259:117764.

Zeb M, Tackaberry LE, Massicotte HB, Egger KN, Reimer K, Lu G, Heiss C, Azadi P, Lee CH. (2021) Structural elucidation and immuno-stimulatory activity of a novel polysaccharide containing glucuronic acid from the fungus Echinodontium tinctorium. Carbohydrate Polymers. 258:117700. Apr 15, 2021.

Mathur B, Shajahan A, Arif W, Chen Q, Hand NJ, Abramowitz LK, Schoonjans K, Rader DJ, Kalsotra A, Hanover JA, Azadi P. (2021) Nuclear receptors FXR and SHP regulate protein N-glycan modifications in the liver. Science Advances. 7(17):eabf4865. Apr 1, 2021.

Shajahan A, Archer-Hartmann S, Supekar NT, Gleinich AS, Heiss C, Azadi P. (2021) Comprehensive characterization of N-and O-glycosylation of SARS-CoV-2 human receptor angiotensin converting enzyme 2. Glycobiology. 31(4):410-24. Apr 2021.

dos Santos Dias L, Dobson HE, Bakke BK, Kujoth GC, Huang J, Kohn EM, Taira CL, Wang H, Supekar NT, Fites JS, Gates D, Gomez CL, Specht CA, Levitz SM, Azadi P, Li L, Suresh M, Klein BS, Wuthrich M. (2021) Structural basis of Blastomyces Endoglucanase-2 adjuvancy in anti-fungal and-viral immunity. PLoS pathogens. 17(3):e1009324. Mar 18, 2021.

Wang M, Shajahan A, Pepi LE, Azadi P, Zaia J. (2021) Glycoproteomic Sample Processing, LC‐MS, and Data Analysis Using GlycReSoft. Current Protocols. 1(3):e84. Mar 2021.

Latchoumane CF, Betancur MI, Simchick GA, Sun MK, Forghani R, Lenear CE, Ahmed A, Mohankumar R, Balaji N, Mason HD, Archer-Hartmann SA, Azadi P, Holmes PV, Zhao Q, Bellamkonda RV, Karumbaiah L. (2021) Engineered glycomaterial implants orchestrate large-scale functional repair of brain tissue chronically after severe traumatic brain injury. Science advances. 7(10):eabe0207. Mar 1, 2021.

Ma X, Li Y, Kondo Y, Shi H, Han J, Jiang Y, Bai X, Archer-Hartmann SA, Azadi P, Ruan C, Fu J. (2021) Slc35a1 deficiency causes thrombocytopenia due to impaired megakaryocytopoiesis and excessive platelet clearance in the liver. haematologica. 106(3):759. Mar 1, 2021.