Collaboration

A major goal of the Analytical Services laboratory at the Complex Carbohydrate Research Center is to stimulate collaboration with members of the broader scientific community. In addition to standard service projects, the Analytical Services laboratory also routinely works with various organizations in the form of research collaborations. This work serves to strengthen the current field of carbohydrate research, as well as to provide the feedback necessary to refine current technology and identify new needs in the field.

Collaboration projects involve our expertise in the fields of plant and microbial polysaccharides, glycomics, glycoproteomics and proteoglycans. These collaborations commonly exploit still-emerging technologies where the projects can be more open-ended and generally require a greater interaction between personnel at the CCRC and outside investigators.

Dr. Parastoo Azadi is the coordinator of Collaborative Endeavors within the CCRC Analytical Services Group. With more than 25 years of experience in the field of polysaccharide and glycoprotein structural analysis, she is well equipped to assist investigators in the development of new collaborative projects with the scientific staff at the CCRC. Initial contact may be made directly with Dr. Azadi at azadi@uga.edu.

Publications resulting from these projects will very likely be co-authored by all those involved in the collaboration, and any publication using data from the Resource should include an acknowledgment similar to:

 

Plant and Microbial Polysaccharide Research

Should any of these data be used in a publication, please include the following statement in the acknowledgment: This work was supported by the Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, U.S. Department of Energy grant (DE-SC0015662) at the Complex Carbohydrate Research Center.

Recent Publications

Ticar BF, Rohmah Z, Neri TAN, Pahila IG, Vasconcelos A, Archer-Hartmann SA, Reiter CEN, Dobruchowska JM, Choi BD, Heiss C, Azadi P, Pomin VH. (2020) Biocompatibility and structural characterization of glycosaminoglycans isolated from heads of silver-banded whiting (Sillago argentifasciata Martin & Montalban 1935). International Journal of Biological Macromolecules, 151 :663-676. May 15, 2020. doi.org/10.1016/j.ijbiomac.2020.02.160

Wolferen M van, Shajahan A, Heinrich K, Brenzinger S, Black IM, Wagner A, Briegel A, Azadi P, Albers SV. (2020) Species-specific recognition of Sulfolobales mediated by UV-inducible pili and S-layer glycosylation patterns. Mbio 11(2):e03014-19. Mar 10, 2020. doi.org/10.1128/mBio.03014-19. PMID: 32156822

Kittur FS, Hung CY, Zhu C, Shajahan A, Azadi P, Thomas MD, Pearce JL, Gruber C, Kallolimath S, Xie J. (2020) Glycoengineering tobacco plants to stably express recombinant human erythropoietin with different N-glycan profiles. Elsevier, 157:158-169. Apr 26 2020. doi.org/10.1016/j.ijbiomac.2020.04.199

Ma X, Li Y, Kondo Y, Shi H, Han J, Jiang Y, Bai X, Archer-Hartmann SA, Azadi P, Ruan C, Fu J, Xia L.(2020) Slc35a1 deficiency causes thrombocytopenia due to impaired megakaryocytopoiesis and excessive platelet clearance in the liver. Haematologica. Apr 2020. doi.org/10.3324/haematol.2019.225987. PMID:32303557

Sacher JC, Shajahan A, Butcher J, Patry RT, Flint A, Hendrixson DR, Stintzi A, Azadi P, Szymanski CM.(2020) Binding of phage-encoded FlaGrab to motile Campylobacter jejuni flagella inhibits growth, downregulates energy metabolism, and requires specific flagellar glycans. Front. Microbiol., 11:397. Mar 20, 2020. doi.org/10.3389/fmicb.2020.00397

Park Y, Kim D, Boorgula GD, Schutter KD, Smagghe G, Šimo L, Archer-Hartmann SA, Azadi P.(2020) Alpha-Gal and Cross-Reactive Carbohydrate Determinants in the N-Glycans of Salivary Glands in the Lone Star Tick, Amblyomma americanum. Vaccines 2020, 8(1):18. Jan 9, 2020. doi.org/10.3390/vaccines8010018

Garber JM, Nothaft H, Pluvinage B, Stahl M, Bian X, Porfirio S, Enriquez A, Butcher J, Huang H, Glushka J, Line E, Gerlt JA, Azadi P, Stintzi A, Boraston AB, Szymanski CM.(2020) The gastrointestinal pathogen Campylobacter jejuni metabolizes sugars with potential help from commensal Bacteroides vulgatus. Commun Biol 3,2(2020), 3(1):1-11. Jan 7, 2020. doi.org/10.1038/s42003-019-0727-5

Lunin V, Wang HT, Bharadwaj V, Alahuhta PM, Pena MJ, Yang JY, Archer-Hartmann SA, Azadi P, Himmel M, Moremen KW, York WS, Bomble YJ, Urbanowicz B.(2020)Molecular mechanism of polysaccharide acetylation by the Arabidopsis xylan O-acetyltransferase XOAT1. The Plant Cell. April 2020.doi.org/10.1105/tpc.20.00028. PMID: 32354790

Wolferen M van, Shajahan A, Heinrich K, Brenzinger S, Black IM, Wagner A, Briegel A, Azadi P, Albers SV.(2020)Species-specific recognition of Sulfolobales mediated by UV-inducible pili and S-layer glycosylation patterns. Molecular Biology and Physiology. doi.org/10.1128/mBio.03014-19.Epub 2020 Mar 10. PMID: 32156822

Urbanowicz B, Lunin V, Wang HT, Bharadwaj V, Alahuhta PM, Pena MJ, Yang JY, Archer-Hartmann SA, Azadi P, Himmel M, Moremen KW, York WS, Bomble YJ.(2020) Structure of the Xylan O-Acetyltransferase AtXOAT1 Reveals Molecular Insight into Polysaccharide Acetylation in Plants. bioRxiv. Jan 17,2020. doi.org/10.1101/2020.01.16.909127

Ge Y, Gong M, Zadeh M, Li J, Abbott JR, Li W, Morel L, Sonon R, Supekar NT, Azadi P, Wang Y, Jones DP, Li S, Mohamadzadeh M.(2020) Regulating colonic dendritic cells by commensal glycosylated large surface layer protein A to sustain gut homeostasis against pathogenic inflammation.Mucosal Immunol 13, 34–46 (2020). doi.org/10.1038/s41385-019-0210-0

De Leoz MLA, Duewer D, Fung A, Liu L, Yau HK, Archer-Hartmann SA, Azadi P, Said N, et al.(2020) NIST interlaboratory study on glycosylation analysis of monoclonal antibodies: comparison of results from diverse analytical methods. Molecular & Cellular Proteomics Jan 1, 2020, First published on Oct 7, 2019, 19 (1).doi.org/10.1074/mcp.RA119.001677. Epub 2020 Jan 1. PMID:31591262