Microbial Carbohydrates

Peptidoglycan Characterization

  • Peptidoglycan characterization – mechanical, chemical  and enzymatic release and purification of murein/peptidoglycan from Gram-positive and Gram-negative bacterial cell;  purification by ultracentrifugation and high speed centrifugation, enzymatic cleavage and FPLC size exclusion, ion exchange and affinity chromatography;  glycosyl composition and linkage analysis of neutral and aminosugar components of PG, structural characterization by high resolution mass spectrometry (MALDI-TOF MS).