Absolute Configuration (D or L) of Monosaccharides
- The absolute configurations of monosaccharides are determined by GC-MS analysis. Identification of D and L forms is made by comparison of retention time to standard D and L monosaccharide.
- Absolute configuration (D or L) of Monosaccharide
- Purpose: Determination of the absolute configuration (D or L) of monosaccharide
- Amount of sample required for analysis: A minimum of 200-500 micrograms of carbohydrate is required, more if available.
- Methods used: The absolute configuration of monosaccharide can be determined by acid-catalyzed derivatization with optically pure 2-butanol, followed by per-O-trimethylsilylation and GC-MS analysis. Identification of the D and L forms is made by comparison of retention times the standard D or L of monosaccharide.