Routine Services

Glycosyl-Linkage Analysis of Polysaccharides and Oligosaccharides

Linkage analysis of polysaccharides and oligosaccharides is performed by partially methylated alditol acetate  (PMAA) method to determine the linkage of monosaccharides and branching points.

Linkage Analysis of Neutral, Uronic Acids or Amino-Residues

(Methylation analysis) – The polysaccharides or oligosaccharides are derivatized prior to GC-MS analysis. This procedure uses either the Hakomori, or NaOH base, for the permethylation procedure.

Linkage Analysis of Kdo

– Kdo (2-keto-3-deoxy-octulosonic acid) is readily analyzed by a modified linkage procedure.

Methylation Method

For glycosyl linkage analysis, the sample is permethylated, depolymerized, reduced, and acetylated; and the resultant partially methylated alditol acetates (PMAAs) are analyzed by gas chromatography-mass spectrometry (GC-MS).

Amount Required for Linkage Sample Analysis

A minimum of 100-200 micrograms of carbohydrate is required, more if available. Your sample must be pure, free of salt and detergent.

Rare Monosaccharide Residues

The CCRC has over 30 years of experience with linkage analysis and has accumulated a vast library of spectra on unusual monosaccharides and their linkages. We have the experience and expertise to determine the glycosyl linkage of any non-routine residue. We can also develop a specific methylation procedure for linkage analysis of your particular glycan if needed.  Glycosyl composition data are usually required prior to glycosyl linkage analysis.