Microbial Carbohydrates

Fatty Acids

  • Total fatty acid analysis – The fatty acids present in a sample are transesterified into their methyl ester forms and are quantitated by GC-MS. The free fatty acids or esters (such as methyl, ethyl, butyl, tocopherol ester) are also analyzed by GC-MS.
  • N- and O-linked fatty acid analysis – Using base mediated hydrolysis to remove O-linked fatty acids and acid mediated hydrolysis to remove all fatty acids, we can determine whether a fatty acid is linked to the saccharide through a hydroxyl or amino group.
Fatty Acids Analysis graph