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National Glycoscience Resource NGR@CCRC
Through the “National Glycoscience Resource” program (NIH grant R24GM137782) we provide services and training for the structural characterization of all types of glycoconjugates to the greater biomedical scientific community including universities, minority serving institutions, federal agencies, biotechnology, and pharmaceutical industries. Our plant characterization projects are supported by an additional grant from the Department of Energy “Center for Plant and Microbial Complex Carbohydrates” (DE-SC0015662). The particular combination of technologies applied for this purpose will depend on the specific structural information desired by the user and will be determined individually after discussion with Dr. Azadi.
NEWS
Exciting collaboration published with Dr. Mougous at Yale University!
Our work explores Streptomyces coelicolor and its “umbrella” toxins, which deploy lectins to recognize distinct sugar patterns on competing bacteria.
We contributed by characterizing a previously undescribed target cell receptor—a unique glycoconjugate composed of a teichuronic acid (TUA)-like polymer linked to wall teichoic acids (WTAs)—using advanced NMR and MS techniques.
Thrilled to share our recent collaboration with Dr. Michael Norris at the University of Hawaii!
“Molecular and structural characterization of a Bacillus cereus strain producing an anthrax-like capsule”
We investigated Bacillus cereus strain PATH2418, responsible for a severe wound infection. While B. cereus is typically harmless, this strain carried genes similar to those found in Bacillus anthracis (the anthrax bacterium).
Genome sequencing revealed the presence of a protective capsule. Our team characterized this capsule through extensive NMR and HPLC analysis, confirming it as a poly-γ-glutamate polymer with the same D/L glutamic acid composition as the anthrax capsule.
New method paper in Analytical Chemistry!
We used HILIC-QTOF-MS + GRIL labeling to quantify GAGs & MPS biomarkers—no ion-pairing needed, better sensitivity!
Big step for glycomics & rare disease research.
Read paper here
New paper out in Carbohydrate Polymers!
We present the most detailed structural analysis of RG-I in Arabidopsis thaliana to date using NMR & MS.
Key findings:
3-O-acetylation on ~10% of Rha residues
Unique β-1,6-galactan terminations
Minor GalA O-acetylation
Read the article
New Publication Alert!
New from our lab: a comprehensive review of methods for determining molecular weight in insoluble polysaccharides—a major analytical challenge. We explore both intact and non-intact strategies, plus the role of molecular modeling.
Read the article
New Publication Alert!
Exciting! Our collaboration with Matt Delissa has been published in Nature Communications!
This study marks a major step forward in antibody engineering—demonstrating how a bacterial enzyme can glycosylate human IgG in E. coli. This breakthrough opens the door to more accessible and cost-effective production of therapeutic antibodies with functional glycosylation. Xu Yang from our group performed glycoproteomic tandem MS analysis of the protein samples. The LC-MS/MS data were instrumental in confirming glycosylation of the native QYNST sequon in the hinge-Fc region by DmPglB.
Read the article
The CCRC Analytical Services Team offers advanced glycomics and glycoproteomics support for biomedical and biotechnological research. Our services include:
- Glycan profiling and sequencing
- Glycoprotein characterization
- Site-specific glycosylation analysis
- Monosaccharide and linkage analysis
- Sialic acid and glycolipid profiling
- Milk oligosaccharide analysis
- Pathogen-related glycomics (SARS-CoV-2, HIV)
We use state-of-the-art techniques including mass spectrometry, NMR, and HPLC to deliver accurate, publication-ready data.
Contact us to learn how we can support your glycomics research.
UPCOMING EVENTS
Registration is Open for 2026 Courses
Course 1 : Techniques For Characterization Of Carbohydrate Structure Of Plant And Microbial Polysaccharides – Polysaccharide Course (August 3-7, 2026)
Course 2 : Separation And Characterization Of Glycoprotein And Glycolipid Oligosaccharides – Glycoprotein Course (August 10-14, 2026)
Course 3 : Analytical Techniques For Structural Analysis Of Glycosaminoglycans (Gags). (August 17-19 2026)
Course 4 : Glycomics, Glycoproteomics And Bioinformatics Tools For Data Interpretation Of Mass Spectrometry Of Glycoproteins (October 12-14, 2026)